LOCUS       40924_35648        3877 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPV2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Hashinaka K.
  TITLE     Synthetic Autonomous Vectors Based on Palindromic Sequences of 
            Parvovirus B19
  JOURNAL   Published Only in Database (2001)
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Hashinaka K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2000) Kazuya Hashinaka, Miyazaki Medical College, 
            Department of Biochemistry; 5200 Kihara, Kiyotake, Miyazaki 
            889-1692, Japan (E-mail:hasinaka@post1.miyazaki-med.ac.jp, 
            Tel:81-985-85-0985, Fax:81-985-85-2401)
REFERENCE   3  (bases 1 to 3877)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3877)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2001)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2000) Kazuya Hashinaka, Miyazaki Medical College, Department
            of Biochemistry"; volume: " 5200 Kihara, Kiyotake, Miyazaki 
            889-1692, Japan (E-mail:hasinaka@post1.miyazaki-med.ac.jp, 
            Tel:81-985-85-0985, Fax"; pages: "81-985-85-2401"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          6..479
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
                     /organism="Human parvovirus B19"
     source          494..977
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
                     /organism="Human parvovirus B19"
     repeat_region   9..391
     repeat_region   586..968
     promoter        complement(986..1004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1011..1027)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1168..1623
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1704..1808
                     /label=AmpR promoter
     CDS             1809..2666
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2840..3428
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3716..3737
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3752..3782
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3790..3806
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3814..3830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3848..3866
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 atgcatggcc aaatcagatg ccgccggtcg ccgccggtag gcgggacttc cggtacaaga
       61 tggcggacaa ttacgtcatt tcctgtgacg tcatttcctg tgacgtcact tccggtgggc
      121 gggacttccg gaattagggt tggctctggg ccagcgcttg gggttgcctt gacactaaga
      181 caagcggcgc gccgcttgat cttagtggca cgtcaacccc aagcaagctg gcccagagcc
      241 aaccctaatt ccggaagtcc cgcccaccgg aagtgacgtc acaggaaatg acgtcacagg
      301 aaatgacgta attgtccgcc atcttgtacc ggaagtcccg cctaccggcg gcgaccggcg
      361 gcatctgatt tggtgtcttc ttttaaattt tagcgggctt ttttcccgcc ttatgcaaat
      421 gggcagccat tttaagtgtt ttactataat tttattggtt agttttgtaa cgaaggtacc
      481 gaattcctcg agtctagtgg gcagatctta agattgtgta atatgtatca aaatttagaa
      541 aaataaacat ttattgtggt taaaaaatta tgttgttgcg ctttaaaaat ttaaaagaag
      601 acaccaaatc agatgccgcc ggtcgccgcc ggtaggcggg acttccggta caagatggcg
      661 gacaattacg tcatttcctg tgacgtcatt tcctgtgacg tcacttccgg tgggcgggac
      721 ttccggaatt agggttggct ctgggccagc ttgcttgggg ttgacgtgcc actaagatca
      781 agcggcgcgc cgcttgtctt agtgtcaagg caaccccaag cgctggccca gagccaaccc
      841 taattccgga agtcccgccc accggaagtg acgtcacagg aaatgacgtc acaggaaatg
      901 acgtaattgt ccgccatctt gtaccggaag tcccgcctac cggcggcgac cggcggcatc
      961 tgatttggcc acgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     1021 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     1081 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1141 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     1201 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1261 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1321 gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc aaaaaacttg
     1381 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1441 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1501 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1561 aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta acgtttacaa
     1621 tttcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacag
     1681 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1741 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1801 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1861 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1921 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1981 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtcatacac
     2041 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgggcgcgg tattctcaga
     2101 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2161 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2221 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2281 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2341 ccacgatgcc tgtagcaatg ccaacaacgt tgcgcaaact attaactggc gaactactta
     2401 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2461 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2521 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2581 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2641 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2701 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2761 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2821 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2881 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2941 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3001 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3061 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3421 tatcgaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3481 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3541 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3601 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3661 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     3721 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     3781 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     3841 ccaagctatt taggtgacac tatagaatac tcaagct
//
